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Lab-on-a-chip Dielectrophoretic Manipulation of Beta-2 Microglobulin for Toxin Removal in An Artificial Kidney

[ Vol. 11 , Issue. 1 ]

Author(s):

Muhammad Izzuddin Abd Samad, Aminuddin Ahmad Kayani, Ahmad Sabirin Zoolfakar, Azrul Azlan Hamzah, Burhanuddin Yeop Majlis and Muhamad Ramdzan Buyong*   Pages 40 - 46 ( 7 )

Abstract:


Background: This paper presents a fundamental study of protein manipulation under the influence of dielectrophoretic (DEP) force for a lab-on-a-chip platform.

Objective: Protein manipulation is dependent on the polarisation factor of protein when exposed to an electric field. Therefore the objective of this work is a microfluidic device and measurement system are used to characterise the human beta-2 microglobulin (β2M) protein via lateral attractive forces and vertical repulsive forces by means of DEP responses.

Method: The manipulation of the β2M protein was conducted using a microfluidic platform with a tapered DEP microelectrode and the protein concentration was quantified based on a biochemical interaction using an Enzyme-Linked Immunosolvent Assay (ELISA). The protein distribution has been analysed based on the β2M concentration for each microfluidic outlet.

Results: At 300 kHz, the protein experienced a negative DEP (nDEP) with of 83.3% protein distribution on the middle microchannel. In contrast, the protein experienced a positive DEP (pDEP) at 1.2 MHz with of 78.7% of protein on the left and right sides of the microchannel.

Conclusion: This is concept proved that the tapered DEP microelectrode is capable of manipulating a β2M via particle polarisation, hence making it suitable to be utilised for purifying proteins in biomedical application.

Keywords:

Dielectrophoresis, Beta-2-microglobulin, Lab on chip, artificial kidney, hollow fibre membrane, end-stage renal disease.

Affiliation:

Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia, Bangi, 43600, Center for Advanced Materials and Green Technology, Multimedia University, 75450 Melaka, NANOElecTronic Centre, NET, Universiti Teknologi Mara, UiTM, Shah Alam 45450, Selangor, Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia, Bangi, 43600, Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia, Bangi, 43600, Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia, Bangi, 43600

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