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Detection of Squamous Cell Carcinoma Antigen with Micro- and Nanogap Interdigitated Electrodes and Gold Nanoparticles

[ Vol. 9 , Issue. 1 ]

Author(s):

Thangavel Lakshmipriya*, Uda Hashim* and C. B. Gopinath   Pages 2 - 6 ( 5 )

Abstract:


Background: Biosensor detects the binding event of biologically relevant molecules, which are analyte and ligand. Showing improved sensitivity is the key and primary characteristic of a high-performance biosensor, which displays lower abundance of an analyte. Here, we preferred the silicon substrate on impedance sensor to demonstrate the improvement in the sensitivity with different band-gaps.

Methods: we have fabricated two band-gaps on the sensing substrate with the sizes of micrometer and nanometer. These gaps were measured to be 85.5 ± 5 µm and 125 ± 3 nm, respectively. Squamous cell carcinoma (SCC) antibody and antigen interaction for the cancer detection was demonstrated on these gaps.

Results: It was found that nano-gap is having higher sensitivity than micro-gap. Further, gold nanoparticle conjugated SCC antigen was found to show the improvement with the micro-gap.

Conclusion: This research has evidenced that lesser band-gap (nano) exhibits a good sensitivity for the detection of SCC antigen rather than micro-gap. Gold nanoparticle conjugation with antigen is suitable for microgaps, whereas no significant improvement with nano-gaps was observed.

Keywords:

Micro-gap, nano-gap, biosensor, SCC-antigen, gold nanoparticles, squamous cell carcinoma, nanometer.

Affiliation:

Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000 Kangar, Perlis, Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000 Kangar, Perlis, Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000 Kangar, Perlis

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